Fig. 2

USP10 interacts with NLRP7 and is highly expressed in CRC. a Protein pulldown experiment for the identification of proteins associated with NLRP7 by incubation of Flag-NLRP7-antibody with protein extracts from CRC cells. The red box indicates one of the most abundant bands as compared with IgG. b IP assay showed that Flag-NLRP7-antibody could pull down USP10 (upper). Correspondingly, USP10-antibody also pulled down NLRP7 (lower). c Immunofluorescence showed colocalization of NLRP7 (red) and USP10 (green). Nuclei were stained with DAPI (blue). Scale bars, 5 μm. d Analysis of USP10 gene expression in CRC from the GEO dataset. e The protein levels of USP10 in CRC tissues and paired normal tissues measured by WB. N: non-tumor, T: tumor. f The USP10 protein in CRC samples and corresponding adjacent normal tissues confirmed by IHC staining. Scale bars, 100 μm. g Correlation analysis of NLRP7 and USP10 protein expression in CRC tissues. ***P < 0.001