Fig. 4

USP10 accelerates the CRC malignant process by upregulating NLRP7. a-c NLRP7 overexpression rescued impaired proliferation, colony formation, migration, and invasive capability caused by USP10 knockdown in HCT116 and SW480 cells. Scale bars, 100 μm. d NLRP7 or USP10 knockdown inhibited the growth of HCT116 tumors in vivo, whereas overexpression of NLRP7 in USP10 knockdown cells had a synergistic effect on tumor growth. e Representative IHC images of Ki-67, E-cadherin, and vimentin in different xenograft sections (magnification: × 600). Scale bars, 50 μm. f Visible metastatic lesions in the liver, and NLRP7 overexpression reversed the inhibition of liver metastasis by USP10 knockdown. g Representative images of hematoxylin-eosin staining sections from metastatic nodules in the liver (magnification: × 200). Scale bars, 25 μm. ***P < 0.001, **P < 0.01, and *P < 0.05