Fig. 8

The SRSF1/circATP5B/miR-185-5p/HOXB5 feedback loop regulated glioma tumorigenesis in vivo. a Representative images showed the size of intracranial tumors in the coronal location of eight groups (negative control, circATP5B knockdown, miR-185-5p mimic, HOXB5 overexpression, SRSF1 overexpression, circATP5B knockdown combined with HOXB5 overexpression, miR-185-5p mimic combined with HOXB5 overexpression, SRSF1 overexpression combined with circATP5B knockdown in GSC406). Scale bar = 10 mm. b The measured tumor volumes among eight GSC406 groups are indicated. (*p < 0.05 vs. the negative control group; ^##p < 0.05 vs. the circATP5B knockdown group, ^$$p < 0.05 vs. the miR-185-5p group, ^&&p < 0.05 vs. the SRSF1 overexpression group; Student’s t-test). c Kaplan-Meier survival curves showed that HOXB5 overexpression, SRSF1 overexpression, circATP5B knockdown combined with HOXB5 overexpression, miR-185-5p mimic combined with HOXB5 overexpression in GSC406 shortened the survival times of nude mice. At the same time, it prolonged the survival times after miR-185-5p mimic was transfected, circATP5B knockdown, and SRSF1 overexpression combined with circATP5B knockdown in GSC406. For each group, n = 5. d Representative immunohistochemical staining showing the changes in HOXB5, SRSF1, IL6, Ki67, CD133 and nestin in the negative control, circATP5B knockdown, miR-185-5p mimic, HOXB5 overexpression, SRSF1 overexpression, circATP5B knockdown combined with HOXB5 overexpression, miR-185-5p mimic combined with HOXB5 overexpression, SRSF1 overexpression combined with circATP5B knockdown group in orthotopic xenograft models. Scale bar = 50 μm. e The German scoring of HOXB5 protein expression in eight groups. (the circATP5B knockdown group vs. the negative control group: p < 0.001; the miR-185-5p mimic group vs. the negative control group: p < 0.001; the HOXB5 overexpression group vs. the negative control group: p < 0.01; the SRSF1 overexpression group vs. the negative control group: p < 0.01; the circATP5B knockdown combined with HOXB5 overexpression group vs. the negative control group: p < 0.01; the miR-185-5p mimic combined with HOXB5 overexpression group: p < 0.01; the SRSF1 overexpression combined with circATP5B knockdown group vs. the negative control group: p < 0.001; Student’s t-test). f Schematic diagram showing that the SRSF1/circATP5B/miR-185-5p/HOXB5 axis promoted glioma proliferation through IL6-mediated JAK2/STAT3 signaling pathway. All data were expressed as the mean ± SD (three independent experiments). EV: empty vector, OE: overexpression, NC: negative control, KD: knockdown. All data were expressed as the mean ± SD (three independent experiments). *p < 0.05; **p < 0.01; ***p < 0.001