Fig. 1

ALG3 was highly expressed in cancerous tissue and radioresistant breast cancer. a mRNA expression level of ALG family were detected by RT-PCR. GAPDH was detected as a loading control in PCR. b Protein expression of ALG3 in radioresistant and radiosensitive breast cancer tissue. α-Tubulin was detected as a loading control in Western blot. c Cell viability after radiation treatment of each cell line was examined by CCK-8 assays. Data were analyzed by Student’s t-test. Each bar represents the mean ± SD of three independent experiments. d, e RT-PCR and Western blot analysis of ALG3 expression in breast cancer cell lines. GAPDH was used as endogenous control in RT-PCR, and α-Tubulin was detected as a loading control in Western blot. Data were analyzed by Student’s t-test. Each bar represents the mean ± SD of three independent experiments. *P < 0.05