Fig. 6

Creatine reduces mitochondrial activity and oxygen consumption, and mitigates ROS production in hypoxic TNBC cells. shNC and shSlc6a8#2 (shSlc) MDA-MB-231 and BT549 cells were cultured under normoxia and hypoxia, and administered 5 mM creatine or PBS, then mitochondrial activity, oxygen consumption and ROS products were assessed. a Mitochondrial membrane potential was determined by JC-1 staining. b, c Mitochondrial complex I and III activities were measured using reagent kit, as elaborated in methods. d, e Oxygen consumption rate (OCR) and basal respiration (complex I mediated OCR) were detected using a Seahorse XF24 Extracellular Flux Analyzer. f ROS level was measured by DCFH-DA staining. Data were presented as mean ± SD (^*P < 0.05, ^**P < 0.01, ^***P < 0.001, ns means no statistical difference)