Fig. 7

The enhanced creatine promotes hypoxic TNBC survival via attenuation of ROS-depended repression of AKT-ERK1/2 signaling. a Ki-67, Bcl-2, Bax and cleaved Caspase-3 (cleaved CASP3) protein levels were detected by western blot in hypoxic TNBC cells supplemented with creatine or NAC. b p-ERK, ERK, p-AKT and AKT protein levels were determined by western blot in hypoxic TNBC cells supplemented with creatine or NAC. c p-ERK, ERK, p-AKT and AKT protein levels were detected by western blot in hypoxic TNBC cells under treatment with creatine alone or combined with AKT inhibitor AZD5363. d Ki-67, Bcl-2, Bax and cleaved Caspase-3 (cleaved CASP3) protein levels were determined by western blot in hypoxic TNBC cells treated the same as e. Data were presented as mean ± SD (^*P < 0.05, ^**P < 0.01)