Fig. 1

ROC1 expression correlated positively with nuclear p65 expression in BCa tissues and associated with a poor prognosis. a Immunostaining with antibodies against ROC1 and p65 (brown) was performed to detect ROC1 and p65 expression in normal bladder urothelial and BCa tissues. TMA sections were counterstained with hematoxylin. Scale bar, 100 μm. b Kaplan–Meier analysis for BCa patients with high ROC1 and nuclear p65 expression, and their association with poor overall survival. P < 0.05, log-rank test. c Linear-regression analysis showed significant a Pearson correlation between ROC1 expression and the mean nuclear p65 IOD/unit area in BCa TMA samples. P < 0.0001 R = 0.428. d Representative images of ROC1 and p65 IHC staining in BCa metastasis and non-metastasis patients. e ROC1 expression in BCa cell lines and NUCs.**P < 0.01 vs Non-metastatic Bca