Fig. 4

Characteristic changes in PSCs induced by NAC treatment in cancer-stroma interaction. PSCs were treated with 2.5 mM NAC, SUIT-2-SN, or SUIT-SN puls NAC, or untreated (Control). a The indicated expression of growth factors and ECM in PSCs were evaluated in qRT-PCR and western blotting. Protein secretion expression levels of growth factors were measured by western blotting of PSC-SN. Protein expression levels of collagen types I, collagen type IV, FN, and α-SMA was measured in whole-cell lysates with β-actin as the Control. b Western blotting of NRF2 and HMOX-1 (two oxidative stress response marker) levels of PSCs treated as above. c Glutathione (GSH) level in PSC cells treated as above. d Intracellular ROS levels in PSCs were treated as above. The values of RFUs (ROS level) were normalized to Control in each PSCs. e Representative microphotograph of α -SMA (Red) and collagen type IV (Green) immunofluorescence staining in PSC1 cells with indicated treatment. Original magnification, × 100. *P < 0.05; **P < 0.01; ***P < 0.001; n.s, no significance