Fig. 2

circ_0072083 silence decreases TMZ resistance in the resistant glioma cells. a circ_0072083 expression was detected via qRT-PCR in resistant cells transfected with sh-circ_0072083 or sh-NC. b IC50 of TMZ was analyzed via CCK-8 in cells with transfection of sh-circ_0072083 or sh-NC. Cell proliferation c, colony ability d, apoptosis e, migration f and g and invasion h were measured by CCK-8, colony formation, flow cytometry, wound healing and transwell analyses respectively in resistant cells transfected with sh-circ_0072083 or sh-NC after treatment of 50 μg/mL of TMZ. i and j Tumor volume and weight were detected in each group (n = 5). Xenograft model was established using U251/TR cells transfected with sh-circ_0072083 or sh-NC, and then treated via PBS or TMZ. k circ_0072083 abundance was measured via qRT-PCR in tumor tissues of each group. ^*P < 0.05