Fig. 2

KLF5 transcriptionally activates BBOX1-AS1 in NSCLC cells. a Online JASPAR software was utilized to predict the possible binding sites of trascription factor KLF5 on BBOX1-AS1 promoter region. b GEPIA database displayed the expression profile of KLF5 in LUSC. c The correlation between BBOX1-AS1 and KLF5 expression was analyzed by bioinformatics tool GEPIA. d qRT-PCR assays were performed to determine the effect of KLF5 overexpression or knockdown on BBOX1-AS1 expression in A549 and SK-MES-1 cells. e ChIP assay was used to examine the binding of KLF5 with BBOX1-AS1 promoter at the predicted sites (P1 and P2). f The schematic diagram of luciferase reporter vectors containging wild type or mutant P2 sequence of BBOX1-AS1 promoter. g Dual luciferase reporter assay was conducted in A549 cells transfected with wt-P2 or mut-P2 reporter and vector or pcDNA-KLF5. h Dual luciferase reporter assay was performed in SK-MES-1 cells transfected with wt-P2 or mut-P2 reporter and si-NC or si-KLF5. ***P < 0.001