Fig. 4

Concentration of active TGF-β1 and total TFG-β1 measured in the cell culture supernatant of PaCa 5061 ITGAV KD cells and control cells using ELISA (a). The proliferation assay showed no significant differences between the ITGAV knockdown groups and the corresponding control groups (b). The static adhesion on fibronectin was reduced by ITGAV knockdown (c). The migratory potential of tumor cells was decreased by the ITGAV KD (d), while the invasive potential was only impaired in the case of BxPC3 cells (e). In the colony-forming assay, BxPC3 ITGAV KD cells formed fewer spheroid colonies than the control cells. PaCa 5061 cells were not able to form colonies in Matrigel after 14 days (f)