Fig. 6

Bcl-XL plays a key role in ZBTB28-induced autophagy. a Expression of Bcl-XL in stable transfected cervical cancer cells. b Structure of wild-type and mutant Bcl-XL reporter plasmid. c % input of Bcl-XL DNA by HA-tag antibody were tested by ChIP-PCR. Then products of ChIP-PCR were used for electrophoresis. d Promoter luciferase activity of wild-type and mutant Bcl-XL in cervical cancer cells. e CaSki and HeLa cells were transfected with Bcl-XL. Sixty hours after transfection, the indicated proteins were detected by western blot after co-immunoprecipitation with BECN1 antibody. *p < 0. 05, **p < 0. 01, ***p < 0. 001