Fig. 4

PUS7 promotes CRC metastasis in a catalytically independent manner. a Images illustrating IHC staining for Ψ in human CRC paired tissues; the corresponding statistical analysis and correlation analysis between PUS7 and Ψ expression are also shown. b Dot plot analysis of the Ψ levels in total RNA extracted from specified stable cell lines. c WB analysis of PUS7 and LASP1 expression in the specified stable cell lines. d IF analysis of LASP1 expression in the specified stable cell lines. e, f Images illustrating Transwell assays in the context of the specified cell lines and the respective statistical analysis. g Images illustrating H&E staining of lung tissues from the diverse groups, the incidence of lung metastasis, and statistical analysis (n = 5 mice per group). h WB analysis of PUS7 and LASP1 expression in the specified stable cell lines. i Images of Transwell assay in the context of the specified cell lines and corresponding statistical analysis. All the data are shown as the mean ± s.d. *P < 0.05, **P < 0.01, ***P < 0.001