Fig. 3

High expression of DEC2 induced dormancy and promoted migratory and invasive abilities of SACC cells. a-d The differences of Ki-67 expression (a), proliferation capacity (b), metabolism (c) and cell cycle (d) between DEC2 over-expressed and vector SACC-83 cells was detected by immunofluorescence, CCK-8 assay, glucose consumption and Flow cytometry, respectively. e and f Cell apoptosis test (e) and senescent analysis (f) in both DEC2 over-expressed and vector cells. g and h Migration and invasion abilities of DEC2 over-expressed/vector SACC-83 cells (g) and SACC-83/SACC-83 + siDEC2 cells (h) were detected by transwell and scratch assays, respectively. i The mRNA levels of HIF1α and P27 in DEC2 overexpression and silence SACC-83 cells were tested by QPCR. Error bars represent the mean ± SD of triplicate experiments. (*P < 0.05, **P < 0.01, ***P < 0 .001)