Fig. 5
From: Inhibition of DEC2 is necessary for exiting cell dormancy in salivary adenoid cystic carcinoma
Slug-induced EMT was involved in DEC2- induced dormancy. a QPCR analyses of NR2F1, P27, P53, HIF1α, CDK4, P38, ERK, E-cadherin, N-cadherin, Snail, Slug, Twist and Zeb in DEC2 overexpressed and vector SACC-83/LM cells. b The protein expression of HIF1α, P27, P53, N-cadherin, Slug, Snail, CDK4 and E-cadherin were detected by western blot in DEC2 overexpressed and vector SACC-83 cells. c Cell morphologies were changed after Slug knockdown in DEC2 overexpressed SACC-83 cells. d and e Cell proliferation (d) and metabolism (e) were determined by CCK-8 assay and glucose consumption in DEC2hi/DEC2hi + siSlug/vector SACC-83 cells. f Transwell chamber and scratch assays for DEC2hi or DEC2hi + siSlug SACC-83 cells. Error bars represent the mean ± SD of triplicate experiments (*P < 0 .05, **P < 0.01, ***P < 0 .001)