Fig. 1

a hematoxylin and eosin staining of the patient surgical specimen showing high-grade polymorphic UPS cells (light blue stroma) infiltrating necrotic tissue, 10 x and 20 x magnification. b hematoxylin and eosin staining of the cytospunned tumor cells from the patient-derived UPS primary culture S1, 10 x and 20 x magnification. c hematoxylin and eosin staining of the cytospunned tumor cells (light blue spots) from the patient-derived UPS primary culture S1 cultured within 3D collagen-based scaffold system, 10 x and 20 x magnification. d Heat map comparisons of the relative gene expression of selected tumor-associated markers tgf-b, slug, snail, mmp9 between UPS patient surgical specimen, S1 patient-derived 2D standard monolayer primary culture and S1 patient-derived 3D primary culture system. e hematoxylin and eosin staining of the patient surgical specimen showing low and high grade UPS and L-sarcoma (light blue stroma) infiltrating adipose tissue, 10x and 20× magnification. f hematoxylin and eosin staining of the cytospunned tumor cells of patient-derived primary cultures UPS and L-sarcoma case series, 10 x and 20 x magnification