Fig. 5
From: Targeting SERT promotes tryptophan metabolism: mechanisms and implications in colon cancer treatment
Endogenous and exogenous serotonin have different subcellular localizations. a SW480 cells were starved with Trp-free and serum-free medium for 24 h after which they were supplied with Trp (75 μM, 9 h) or serotonin (50 μM, 4 h), respectively. Serotonin localization was visualized by IF staining with anti-serotonin (green). DNA was stained with DAPI (blue). Scale bar: 25 μm. b SW480 cells were transfected with siSERT (48 h) or negative control (48 h) or sertraline (15 μM, 12 h). Serotonin localization was visualized by IF staining with anti-serotonin (green). DNA was stained with DAPI (blue). Scale bar: 25 μm. c SW480 cells were transfected with lyso-EGFP (12 h) or the negative control (12 h) and starved with FBS-free medium (24 h) followed by serotonin (50 μM) stimulation (4 h) . Serotonin localization was visualized by IF staining with anti-serotonin (red) while lysosomal localization was visualized as green. DNA was stained with DAPI (blue). Scale bar: 25 μm. d SW480 cells were transfected with lyso-EGFP (24 h) or negative control (24 h) and then treated with sertraline (15 μM) or MDC (200 μM) for 30 min. Serotonin localization was visualized by IF staining with anti-serotonin (red) while lysosome localization was visualized as green. DNA was stained with DAPI (blue). Scale bar: 25 μm