Fig. 6

DLX6-AS1 interacts with miR-15a-5p. a. RT-qPCR detection of miR-15a-5p expression in cancer tissues and normal tissues; b. RT-qPCR detection of miR-15a-5p expression in cancer cells and normal liver cells; c. RT-qPCR detection of miR-15a-5p expression in macrophages after co-culture with HCC-exo delivering oe-DLX6-AS1; d. Prediction of the potential binding sites of DLX6-AS1 and miR-15a-5p through bioinformatics websites; e. Verification of the targeting relationship between DLX6-AS1 and miR-15a-5p through dual luciferase reporter experiment; f. RIP detection of the binding relationship between DLX6-AS1 and miR-15a-5p; g. RNA pull-down detection of the binding relationship between DLX6-AS1 and miR-15a-5p; data were expressed as mean ± standard deviation (repetition = 3) and evaluated by One-way AVONA and Tukey’s test. * P < 0.05 compared with the oe-NC-exo group