Fig. 6

CCNF promotes LUSC cell proliferation. a qRT-PCR and western blot analysis of CCNF expression in H520 cells after treatment with si-CCNF, NC, CCNF vector or mock; b Assessment of proliferation in H520 and H2170 cells transfected with si-CCNF, NC, CCNF vector or mock by CCK-8 assay; c Assessment of DNA synthesis using an EdU assay in H520 and H2170 cells transfected with s si-CCNF, NC, CCNF vector or mock. Scale bar = 200 μm; d Colony formation assay in H520 and H2170 cells transfected with si-CCNF, NC, CCNF vector or mock; e IHC analysis of expression levels of CCNF between LUSC tissues and matched adjacent non-tumor tissues.; f Relative expression of CCNF in LUSC tissues compared to normal tissues analyzed using TCGA data; g Assessment of proliferation in H520 cells after 72 h transfected with CCNF siRNA or miR-30d inhibitors or miR-30d inhibitors by CCK-8 assay. Data are shown as mean ± SD, *P < 0.05; **P < 0.01; ***P < 0.001