Fig. 1

SRSF1 is upregulated in breast cancer samples and high expression of SRSF1 correlates with aggressive clinical characteristics and a poor prognosis in hormone receptor positive (HR+) subgroups. a The volcanic map and heatmap of differentially expressed SR family proteins in breast cancer samples (filtered criteria: |logFC| > =0.5, FDR < 0.05). b Scattered plots comparing SRSF1 mRNA expression levels in normal and tumor samples in TCGA dataset; Data are presented as mean ± SD; Statistical significance is calculated by unpaired t-test. c Correlation between relative expression of Ki67 and SRSF1 using Pearson correlation test based on TCGA dataset downloaded from GEPIA website. d Correlation between RNA expression levels of SRSF1 and histological grade based on Metabric dataset downloaded from cBioPortal using One-way ANOVA test. e Kaplan-Meier plots of ER/PR+ subgroup patients with high and low SRSF1 expression. f Kaplan-Meier plots of HR- subgroup patients with high and low SRSF1 expression. g Representative immunostaining images of SRSF1 in tumor and adjacent breast tissues (200*). h Scattered plots of SRSF1 protein expression assessed by blinded IHC analysis of tumor (n = 42) and adjacent breast tissues (n = 25). i Scattered plots of SRSF1 mRNA expression in tumor and paired adjacent normal tissues (n = 38;****, P < 0.0001; paired t-test)