Fig. 6

PTPMT1 splicing switch are required for cell growth and migration in vitro and in vivo. a qRT-PCR for testing the inhibitory effects of siPTPMT1-1/2 specific targeting to PTPMT1-L in MCF7/MDA-MB-231 cell lines. b Cell proliferation assay is performed using cells described in (a). c Clonogenic survival assay for control (pLKO.1) and treated (shPTPMT1-L) cells are conducted and then quantified in the bar graph. d qRT-PCR for testing overexpressing efficiency of PTPMT1-L/S plasmids in MCF7/MDA-MB-231 cell lines. e, f Cell proliferation assay and clonogenic survival assay for control (pCMV) and overexpression (PTPMT1-L/S) cells. g Measurement of cell migration by transwell assays using cells described in (d). h MDA-MB-231/PTPMT1-L/S cells or control MDA-MB-231/pCMV cells are transplanted to nude mice. Tumors derived from MDA-MB-231/PTPMT1-L/S or control cells are excised and presented. i Weight of tumors are calculated and plotted. j Representative IHC results of Ki67 and MYC in xenografts are presented (200*). Unpaired t-test. *, P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001