Fig. 7
From: Splicing factor SRSF1 promotes breast cancer progression via oncogenic splice switching of PTPMT1
PTPMT1-L partially recapitulates the SRSF1-mediated tumor-promoting phenotypes through the P-AKT/C-MYC signaling pathways in breast cancer cells. a, b Cell proliferation assay (a) and clonogenic survival assay (b) of MCF7/MDA-MB-231 cells with depletion of SRSF1 or control, and re-expression of PTPMT1-L in SRSF1depleted cells are performed. c Measurement of cell migration by transwell assays using MCF7/MDA-MB-231 cells with depletion of SRSF1 or control, and re-expression of PTPMT1-L in SRSF1depleted cells. d Western blot for testing PI3K/AKT/C-MYC signaling pathways in MCF7/T47D/MDA-MB-231 cells with depletion of SRSF1 or control. e Western blot for testing PI3K/AKT/C-MYC signaling pathways in MCF7/T47D/MDA-MB-231 cells with depletion of PTPMT1-L or control. f Western blot for testing PI3K/AKT/C-MYC signaling pathways in MCF7/T47D/MDA-MB-231 cells with PTPMT1-L/S overexpression or control. g Western blot for testing PI3K/AKT/C-MYC signaling pathways in MCF7/T47D/MDA-MB-231 cells with depletion of SRSF1 or control, and re-expression of PTPMT1-L or control vector in SRSF1 depleted cells