Fig. 1

Schematic representation of the experimental workflow. A Patient-derived colon organoids (PDCOs) were generated from 3 patients affected by CRC. The crypts derived from normal tissue and the isolated cells from tumor were used to generate normal and tumor PDCOs, respectively. Normal PDCOs were grown in medium added with the Wnt3a factor (+W), whereas tumor PDCOs in medium in the presence (+W) or absence of the Wnt3a factor (−W). PDCOs’ genomic DNA (gDNA), RNA and protein were collected and were analyzed by whole-exome sequencing (WES), microsatellite profile, Targeted NGS, RNA-seq, reverse-phase protein microarrays (RPPA) and histochemistry. B A scheme of the experimental settings for each type of analysis is indicated