Fig. 4

LINC00511 controls MVB targeting to the plasma membrane by regulating RAB27B expression and localization. a PCR detected the mRNA levels of RAB5, RAB7, RAB11, RAB27A, RAB27B, and RAB35 in LINC00511 overexpressing Huh7 cells. b Immunoblotting analysis of RAB27B expression from the LINC00511 overexpressing Huh7 and Hep3B cells. c Quantification of RAB27B levels for each condition in (B). d A total of 30 pairs of HCC (T) and para-tumor (N) liver tissues were included. IHC scores for RAB27B were determined. Bars: (left) scale bars, 1.25 mm, (right) scale bars,100 μm. e Upper graph: RAB27B expression in HCC from TCGA (T = 374 cases, N = 50 cases). Lower graph: RAB27B expression in the high expression of LINC00511 group in HCC from TCGA. f Immunofluorescence of CD63 (green) and RAB27B (red) distribution in Huh7 cells transfected with Ctrl or LINC00511. Scale bar, 10um. g Colocalization analysis of cells stained for CD63 and RAB27B. h Immunofluorescence of CD63 (green) and RAB27B (red) distribution in HCC and adjacent tissues. Scale bars,10um. i Immunoblotting analysis of RAB27B expression from transfected huh7 cells with si-RAB27B. j Quantification of concentration of exosome release in Huh7 cells co-transfected with LINC00511 and si-RAB27B. Data are mean ± SD from three independent experiments, t-test *, P < 0.05, **, P < 0.01