Fig. 5

LINC00511 induces hepatocellular carcinoma cell invasion and invadopodia formation. a Enrichment plot showing enrichment of ECM-related genes in the LINC00511 high expression group. b, c Huh7 and Hep3B cells transfected with Ctrl or LINC00511 were seeded on Matrigel-coated cell inserts and allowed to invade for 24 h, and then invasive cells were counted. Scale bar, 200 um. d, e Representative images and quantification of the invasive ability of Huh7 and Hep3B cells transfected with Ctrl or LINC00511. After 48 h of invasion, serial optical sections (10 μm interval) were acquired. Red arrow indicates the direction of invasive movement. Scale bar, 100 μm. f Huh7 cells transfected with Ctrl or LINC00511 were stained for invadopodia markers cortactin (green) and actin (Phalloidin-iFluor 594). Images were analyzed on the confocal microscope and the percentage of cells forming invadopodia was quantified and plotted. Scale bar,10 μm. g, h Immunofluorescence of in HCC and adjacent tissues stained with invadopodia markers cortactin (green) and actin (Phalloidin-iFluor 594). Scale bar, 20 μm. Data are mean ± SD from three independent experiments, t-test *, P < 0.05, **, P < 0.01