Fig. 2

Silencing of CD151 inhibits NSCLC cell proliferation, migration and invasion. a CD151 mRNA and protein levels in NSCLC cell lines which transfected with either CD151 shRNAs (sh-CD151-1 and sh-CD151-2) or negative control (sh-NC). b CCK-8 assay of cell viability in NSCLC cell lines. c Representative images of clonogenic analysis of cell proliferation in NSCLC cells. Bar charts showed clonogenic growth of NSCLC cells. d EdU incorporation assay for cell proliferation (n = 3). EdU (red), DAPI (blue). Scale bar = 200 μm. e Wound healing assay was performed to observe the role of CD151 in A549 and H1299 cells. f Representative images of the transwell assay results for cell migration and invasion in A549 and H1299 cells (sh-CD151 compared with sh-NC). β-actin was used as the internal control. Bars represent mean ± SD from three independent experiments. Significant differences compared with the control: * P < 0.05; **P < 0.01; ***P < 0.001