Fig. 1

Characterization of the new GAC cell lines and in vitro growth features. A & B. Cell morphology of three new patient-derived GAC cell lines (GA0518, GA0804, and GA0825) was observed under a bright-field (BF) microscope (A), and cells stably transfected with mCherry-Luciferase were observed under immunofluorescence microscope (B). C. Doubling time for each of the three cell lines was estimated at the exponential phase according to Materials and Methods. D. Karyotyping analyses of GA0518 and GA0804 cells were performed by the Cytogenetics and Cell Authentication Core at MD Anderson as described in Materials and Methods. Abnormal chromosomal changes were observed in GA0518 (left) and GA0804 cells (right). Red arrows indicate some of the chromosomal changes