Fig. 2

Morphological evidence of the interaction between USP1 and RPS16. a HepG2 and HCCLM3 cells were transfected with FLAG-USP1 plasmids for 48 h. Cell immunofluorescence assay was performed using FLAG-tag and RPS16 antibodies. b HepG2 and HCCLM3 cells were exposed to ML323 for 24 h. Cell immunofluorescence assay was performed using RPS16 antibodies. Representative images are shown. Scale bars, 10 μm. c Quantification of immunofluorescence assay in HCC cells