Fig. 2

HK2 promotes the EMT phenotypes in EC cells. a qRT-PCR analysis of HK2 expression in EC cells after overexpression or knockdown of HK2. b Western blotting analysis of HK2, E-cadherin (CDH1), and Vimentin expression in EC cells transfected as indicated. c HK2 affects EC cell morphology. d, e, f Migration (d), invasion (e), and sphere formation (f) of EC cells after overexpression or knockdown of HK2. g Cell survival was examined by a cell viability assay in EC cells transfected as indicated and treated with TX. h A heat map of qRT-PCR results shows the expression of the indicated genes in EC cells upon overexpression or knockdown of HK2. Red: upregulation; blue: downregulation. Ishi: Ishikawa; vec: vector; sh: shRNA. *P < 0.05