Fig. 2

tiRNA-Gly regulates malignant activities of PTC cells in vitro and in vivo. A Relative expression of tiRNA-Gly in three human PTC cell lines detected by qRT-PCR. B Overexpression efficiency of tiRNA-Gly transfection in K1 cells by qRT-PCR. C-D tiRNA-Gly overexpression promotes K1 cells proliferation and migration by CCK-8 assay and migration assay compare to scramble sequence (SCR). Scale bar = 100 μm. E Knockdown efficiency of si-tiRNA-Gly on tiRNA-Gly and total tRNA-Gly in TPC-1 and BCPAP cells by qRT-PCR. F-G tiRNA-Gly knockdown suppresses TPC-1 and BCPAP cells proliferation and migration by CCK-8 assay and migration assay compare to scramble sequence (SCR). Scale bar = 100 μm. H-J Tumor growth in si-tiRNA-Gly group (n = 10) was suppressed in tumor volume and tumor weight compared with NS group (n = 10). K Ki-67 staining of tumors in si-tiRNA-Gly group is downregulated compared with NS group. Scale bar = 20 μm. Values are triple replicated and displayed in mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001