Fig. 4

RBM17 promotes cell proliferation and migration of PTC cells. A-B RBM17 expression in three human PTC cell lines by qRT-PCR and western blot. β-actin acts as control. C-D Overexpression efficiency of RBM17 in K1 cell transfected with pCMV-RBM17 plasmid by qRT-PCR and Western blot with its quantification result. β-actin referred as a control. Negative control (NC) means control group. E-F RBM17 overexpression promotes proliferation and migration of K1 cells by CCK-8 assay and migration assay compare to vector group (NC). Scale bar = 100 μm. G-H Knockdown efficiency of RBM17 in TPC-1 and BCPAP cells transfected with siRNA-RBM17 by qRT-PCR and western blot with its quantification result. β-actin referred as a control. I-J RBM17 knockdown suppresses proliferation and migration of K1 cells by CCK-8 assay and migration assay compare to vector group (NC). Scale bar = 100 μm. K RBM17 expression in tumor (T) is higher than in adjacent tissues (AT) in 91 pairs PTC patients by IHC. Scale bar = 20 μm. Values are triple replicated and displayed in mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001