Common and mutation specific phenotypes of KRAS and BRAF mutations in colorectal cancer cells revealed by integrative -omics analysis

Table 1 Knock-in cell models and isogenic control cells. Knock-in cell models were generated with rAVV mediated gene editing. Mutation status for KRAS and BRAF oncogene genes in each of the isogenic knock-ins and control cell lines were validated by Sanger sequencing

Designations	Clone	BRAF c.1799T> A (V600E)	BRAF genotype	KRAS				KRAS genotype	Reference
Designations	Clone	BRAF c.1799T> A (V600E)	BRAF genotype	c.38G>A (G13D)	c.34G>T (G12C)	c.35G>T (G12V)	c.35G>A (G12D)	KRAS genotype	Reference
HCT116	Parental	T	wt	G/A	G	G	G	wt/G13D	(Brattain et al. 1981 [22])
HCT116 KRAS WT	DK(HD)	T	Wt	G	G	G	G	wt/KO	(Shirasawa et al. 1993) [24]
HCT116 BRAF V600E	MHB3.2	T/A	wt/V600E	G	G	G	G	wt/KO	This work
RKO	Parental	T/A/A	wt/V600E/ V600E	G	G	G	G	wt/wt	(Brattain et al. 1984 [23])
RKO BRAF WT	RB(HD)	T	wt/KO/KO	G	G	G	G	wt/wt	(Yun et al. 2009) [5]
RKO KRAS G13D	MH2.1	T	wt/KO/KO	G/A	G	G	G	wt/G13D	This work
RKO KRAS G13D	MH3.1	T	wt/KO/KO	G/A	G	G	G	wt/G13D	This work
RKO KRAS G12C	MHC1.1	T	wt/KO/KO	G	G/T	G	G	wt/G12C	This work
RKO KRAS G12C	MHC2.1	T	wt/KO/KO	G	G/T	G	G	wt/G12C	This work
RKO KRAS G12V	MHV1.1	T	wt/KO/KO	G	G	G/T	G	wt/G12V	This work
RKO KRAS G12V	MHV2.2	T	wt/KO/KO	G	G	G/T	G	wt/G12V	This work
RKO KRAS G12D	MHD2.1	T	wt/KO/KO	G	G	G	G/A	wt/G12D	This work
RKO KRAS G12D	MHD1.1	T	wt/KO/KO	G	G	G	G/A	wt/G12D	This work

ISSN: 1756-9966