Fig. 2

The expression of circ_0084615 in CRC cells and the features of circ_0084615. (A) The expression of circ_0084615 in FHC, SW620, DLD1, SW480 and HT-29 cells was detected by qRT-PCR assay. (B) Circ_0084615 was formed by gene ASPH. (C) The existence of CIRC_0084615 was detected by qRT-PCR assay with divergent or convergent primers. (D and E) The existence of circ_0084615 in the reverse transcription products using random hexamer primers and oligo (dT)18 primers. (F and G) The levels of circ_0084615 and ASPH in SW620 and DLD-1 cells treated with Act D were examined with qRT-PCR assay. (H and I) The levels of circ_0084615 and ASPH in SW620 and DLD-1 cells treated with or without RNase R were detected by qRT-PCR assay. (J and K) The expression of circ_0084615 in the cytoplasm and nucleus of SW620 and DLD-1 cells was analyzed with subcellular fraction analysis. **P < 0.01, ***P < 0.001