Table 1 CK2-phosphorylated Che-1 residues identified by nano-LC-mass spectrometry. MS/MS analysis of an in vitro phosphorylation assay showing Che-1 phosphopeptides including Ser316, Ser320 and Ser321. For each peptide the following details are reported: aminoacid sequence, modifications, phosphorylation site(s), pRS peptide score, pRS isoform probability, pRS site probability, q-value, PEP score and number of missed-cleavages. For details about the meaning of these parameters see Taus et al. [31]
From: CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity
Sequence | Modifications | Phospo-site | pRS Score | pRS Probability % | pRS Site Probabilities % | q-Value | PEP | # Missed Cleavages |
|---|---|---|---|---|---|---|---|---|
TPGFSVQSIsDFEK | S10(Phospho) | S155 | 228 | 100.0 | S (5): 0.0; S (8): 0.0; S (10): 100.0 | 0 | 1.8 × 10− 5 | 0 |
YLVDGTKPNAGSEEISsEDDELVEEK | S17(Phospho) | S320/S321 | 144 | 50.0 | S (12): 0.0; S (16): 50.0; S (17): 50.0 | 0 | 4.3 × 10− 5 | 0 |
YLVDGTKPNAGsEEISSEDDELVEEKK | S12(Phospho) | S316 | 124 | 95.9 | S (12): 95.9; S (16): 2.1; S (17): 2.1 | 0 | 9.3 × 10− 4 | 1 |
sLVGLqEELLFqYPDTR | S1(Phospho); Q6(Deamidated); Q12(Deamidated) | S288 | 95 | 100 | S (1): 100.0 | 0 | 5 × 10− 4 | 0 |