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Table 1 CK2-phosphorylated Che-1 residues identified by nano-LC-mass spectrometry. MS/MS analysis of an in vitro phosphorylation assay showing Che-1 phosphopeptides including Ser316, Ser320 and Ser321. For each peptide the following details are reported: aminoacid sequence, modifications, phosphorylation site(s), pRS peptide score, pRS isoform probability, pRS site probability, q-value, PEP score and number of missed-cleavages. For details about the meaning of these parameters see Taus et al. [31]

From: CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity

Sequence Modifications Phospo-site pRS
Score
pRS
Probability %
pRS Site Probabilities % q-Value PEP # Missed Cleavages
TPGFSVQSIsDFEK S10(Phospho) S155 228 100.0 S (5): 0.0; S (8): 0.0; S (10): 100.0 0 1.8 × 10− 5 0
YLVDGTKPNAGSEEISsEDDELVEEK S17(Phospho) S320/S321 144 50.0 S (12): 0.0; S (16): 50.0; S (17): 50.0 0 4.3 × 10− 5 0
YLVDGTKPNAGsEEISSEDDELVEEKK S12(Phospho) S316 124 95.9 S (12): 95.9; S (16): 2.1; S (17): 2.1 0 9.3 × 10− 4 1
sLVGLqEELLFqYPDTR S1(Phospho); Q6(Deamidated); Q12(Deamidated) S288 95 100 S (1): 100.0 0 5 × 10− 4 0