Fig. 4

REG and REG + aPD1 significantly reduce intratumoral macrophages and Treg cells. a Staining of frozen tumor sections for macrophages (F4/80) and quantification: mean values ± SD (n = 5–7) and individual values (dots). b Representative immunofluorescence images. Left panel: staining for F4/80 in green; right panel: staining for FoxP3 in red. Nuclei are DAPI stained (blue). Scale bars: 100 µm. c Quantification of staining for Treg cells (FoxP3) on day 14 with mean values ± SD (n = 6–7) and individual values (dots) and ratio of CD8⁺ cytotoxic T cells to Treg cells (see Supplementary Figure S3a for quantification for CD8⁺). Statistical significance (^§day 7; ^#day 11; * day 14): *^/§/#p < 0.05; **^/§§/##p < 0.01; ***^/§§§/###p < 0.001; ****^{/§§§§/####}p < 0.0001