Fig. 2

XPO1 inhibitor KPT-335 inhibits neuroblastoma cells proliferation by promoting cell apoptosis. A Proliferation inhibition of SK-N-BE(2) and SH-SY5Y by selective inhibitor KPT-335 in dose and time-dependent manners. B Inhibition of colonies formation after KPT-335 treatment in SK-N-BE(2) and SH-SY5Y. C Fluorescence images of EdU-488 after KPT-335 treatment in SK-N-BE(2) and SH-SY5Y. D knockdown of XPO1 by siRNA inhibited SK-N-BE(2) and SH-SY5Y proliferation. E KPT-335 induced SK-N-BE(2) and SH-SY5Y cells apoptosis. F Fluorescence images of TUNEL after KPT-335 treatment in SK-N-BE(2) and SH-SY5Y. G Bad, Bax, Bcl-2 and XPO1 expression levels detected by western blotting. TUNEL, TdT-mediated dUTP Nick-End Labeling assays. Data presented as mean ± SD (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001