Fig. 4
From: Metabolic impairment of non-small cell lung cancers by mitochondrial HSPD1 targeting
The small HSPD1-targeting molecule KSH101 induces cell growth arrest. A) Real-time proliferation curves of A549, Calu-1, H1299 and H460 treated either with vehicle (DMSO) or KHS101. P-values are from two-way ANOVA. Points are average of biological replicates ± SD. **** < 0.0001. B) Images of KHS101 and HB072 synthetic molecular structures. C) Real-time proliferation curves of Calu-1 cells treated either with KHS101 or with the corresponding inactive KHS101 analog (HB072) compared to vehicle-treated cells. P-values are from two-way ANOVA. Points are average of biological replicates ± SD. **** < 0.0001. Colony formation of H460 (D) and H1299 (E) treated for 5 days with KHS101 or vehicle and then left until forming visible colonies in drug-free media, then stained with crystal-violet and quantified in triplicates. Bars are average values of biological replicates ± SD. P-values are from unpaired t-test. *** < 0.001, **** < 0.0001. FACS plots of A549 (F) and H460 (H) treated 24 h with KHS101 and stained with PI for cell cycle analysis. Bar graph showing the % of cells in each cell cycle phase of A549 (G) and H460 (I) cells treated 24 h with vehicle or KHS101. P-values are from two-way ANOVA. Bars are average of biological replicates ± SD. ** < 0.01, **** < 0.0001