Fig. 1

Characterization of COX-2 and CYP19A1 in MPM tissues and cell lines. A The graph represents the correlation between COX-2 and CYP19A1 staining in 29 MPM specimens. The score indicates the intensity of COX-2 and CYP19A1 staining: 0, absent, 1, low; 2, high. B The graph represents the means ± SD of three independent quantifications by RT-PCR of COX-2 and CYP19A1 mRNA levels in Ist Mes1, Ist Mes2, MPP89, NCIH-2452 (NCI), and MSTO-211 H (MSTO) cells. C Graphical representation of Spearman’s correlation between COX-2 and CYP19A1 mRNA levels in MPM cells D Representative experiment out of 3 independent western blots on COX-2 and CYP19A1 expression in MPM cell lines; E The graph represents the average of 3 experiments for the detection of COX-2 and CYP19A1 expression in the five MPM cell lines by western blot. Protein expression was measured by Scion Image Software as the intensity of each band relative to the loading control (relative band intensity). F Graphical representation of Spearman’s correlation between COX-2 and CYP19A1 protein expression in MPM cells.*, statistically significant effects (paired Student t test P < 0.05) compared to CNTR