Fig. 5

Role of EGFR and TGFα in the proliferation of kidney cancer cell lines. A. Knockdown of EGFR and D. TGFα in renal cancer cell lines. Cells were infected with control vector (pLKO) and viruses including two different short hairpin sequences targeting EGFR (A) and TGFA (D). Cell extracts were obtained and the receptor expression was measured by immunoprecipitation followed by western blot with the specific antibody. B. EGFR or E. TGFα knockdown effect on the proliferation of renal cancer cells. Cells were infected with the indicated shRNAs. After selection, cells were then plated and counted after 5 days. Results are plotted as the mean ± s.d. of triplicates with respect to the proliferation of cultures infected with the control vector. C. Effect of cetuximab on the proliferation of renal cancer cells. Cells were treated with 10 nM of cetuximab or anti-human IgG antibody (hIgG) for 5 days and then counted. Graph bars represent the mean ± s.d. of triplicates normalized to cells treated with hIgG as a control. F. Effect of a neutralizing anti-TGFα antibody on the proliferation of renal cancer cells. Cells were treated as in C. and counted