Fig. 2

Disruption of tissue-specific Pten or Tp53 expression in larvae and mature zebrafish. a Representative images of whole-mount in situ hybridization using an anti-sense RNA probe against Cas9 mRNA in 3 dpf WT and fabp10^WT larvae (black arrows). Scale bars, 500 μm. b, c Representative images of mCherry (red) and endogenous Tp53 or Pten (green, FITC-labelled) expression in liver tissues of 1-month-old tp53 KO, pten KO, and fabp10^WT control fish. Scale bars, 50 μm