Fig. 6

Blockade of Akt activation inhibits hepatocarcinogenesis in zebrafish. a Expression levels of Pten, Akt, and pAkt in liver tissues from 1-month-old fabp10^WT, pten KO and pten;tp53 cKO fish. b Representative histological images of liver tissues from pten KO and pten;tp53 cKO fish treated with or without MK-2206. Scale bar, 100 μm. c Immunoreactivity of pAkt, Pcna, pHH3, and Gs in liver tissues from pten KO and pten;tp53 cKO fish treated with or without MK-2206. Scale bars, 100 μm. d Quantification of the percentages of Pcna-positive cells in liver tissues from pten KO and pten;tp53 cKO fish treated with or without MK-2206 (n = 10 random fields of view). e The tumour incidences were significantly suppressed by MK-2206 treatment in 3-month-old pten KO and pten;tp53 cKO fish. The data are shown as the mean ± SEM values