Fig. 6

NACC1 overexpression restrained the suppression effect of PDAC by circRHOBTB3 knockdown. a The efficiency of NACC1 siRNA and overexpression vector is measured by qRT-PCR in PANC-1. b The protein levels of NACC1 knockdown and overexpression were determined by Western Blotting. c-e The retarded proliferation ability caused by circRHOBTB3 was reversed by NACC1 overexpression in PANC-1 cells in CCK-8, EdU incorporation and colony formation assays. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001