Fig. 7

NACC1 overexpression reversed the inhibiting effect on PANC-1 cells autophagy by circRHOBTB3 knockdown. a mCherry-GFP-LC3B labeled PANC-1 cells were infected with circRHOBTB3 knockdown lentivirus and NACC1 overexpression vector, and the autophagy flux was analyzed by confocal microscopy. Representative confocal microscopy images and quantitative data were shown. Scale bar, 20 μm. b The autophagosome in PANC-1 cells were detected by transmission electron microscope (TEM) upon circRHOBTB3 knockdown and NACC1 overexpression and corresponding negative control groups. The samples were imaged at 6000X and 15,000X magnification. Arrows indicate autophagosomes in cells. c-d The protein levels of LC3B II, p62, phosphorylated AKT(p-AKT(T308), p-AKT(S473)), and phosphorylated mTOR(p-mTOR) in PANC-1 cells with circRHOBTB3 knockdown upon NACC1 overexpression. All data are presented as the means ± SD of three independent experiments. *p < 0.05, **p < 0.01