Fig. 3
From: Neuromedin U induces an invasive phenotype in CRC cells expressing the NMUR2 receptor
NMU secretion by colorectal cancer cells. A Secreted NMU was immunoprecipitated from the conditioned media (CM) of CRC cells and analysed by immunoblotting. The image shows representative immunoprecipitation analysis results. B Immunoprecipitated proteins from the CM of HCT116 cells were excised from the gel and subjected to mass spectrometry analysis. PEAKS X Studio analysis of the resulting spectra indicated that two types of NMU isoforms were secreted based on the detection of unique peptides. C Extracellular vesicles released from HCT116 cells were isolated from the conditioned media and lysed, and the levels of NMU, EV markers and EV purity indicators were analysed by immunoblotting (n = 3)