Fig. 5

Circ-GALNT16 promotes the interaction between hnRNPK and p53 via inhibiting SENP2-mediated deSUMOylation. a Co-IP assay was performed in protein lysate pulled down by circ-GALNT16 specifical probe using anti-hnRNPK. b SUMOylation modification analysis was performed to identify the levels of hnRNPK SUMOylation in circ-GALNT16 knockdown and overexpression cells at 6 h after UV stimulation. c The co-IP assay was performed between SENP2 and hnRNPK in circ-GALNT16 silencing and overexpression cells. d SUMOylation modification analysis to explore the levels of hnRNPK SUMOylation in circ-GALNT16 knockdown and SENP2 knockdown cells at 6 h after UV stimulation. e The co-IP assay elucidated that circ-GALNT16 could mediate the interaction between hnRNPK and p53. f Three modes of circRNA-protein interactions. g RIP assay showed p53 did not interact with circ-GALNT16 directly. h Co-IP assay between p53 and hnRNPK applied in circ-GALNT16 and SENP2 depletion cells. All data are presented as the means ± SD of three independent experiments. ^nsp > 0.05, **p < 0.01