Fig. 3

CIRP Promotes the Aggression of NSCLC Cells. (A) Representative microphotographs showing the migration of A549 and H460 cells in which CIRP expression was silenced by siRNA. The migrated cell numbers are shown in histograms. (B) Representative microphotographs showing the migration of CIRP-overexpressing H1299 and H1650 cells. The migrated cell numbers are shown in histograms. (C) Representative microphotographs showing the invasion of A549 and H460 cells in which CIRP expression was silenced by siRNA. The invaded cell numbers are shown in histograms. (D) Representative microphotographs showing the invasion of CIRP-overexpressing H1299 and H1650 cells. The invaded cell numbers are shown in histograms. All data are the means ± SD from three independent experiments (**, P < 0.01). (E) Luciferase images of mice at 7-, 14-, and 21-days post-tail intravenous injection with Le-shCIRP or Le-Scrambled -infected A549-Luc⁺ (n = 6, per group). (F) The average bioluminescence signals in the Le-shCIRP group at different time points are compared with those in the A549-Luc⁺ cells transduced with the Le-Scrambled group (mean ± SD; n = 6, **, P < 0.01). (G) Survival analysis of mice implanted with A549-Luc⁺ cells with or without CIRP knockdown