Fig. 5
CIRP Regulates the Wnt/β-catenin Signaling Pathway in NSCLC Cells. (A) The top 10 enriched pathways affected by CIRP downregulation in A549 cells are shown. (B) Heat map showing the mRNA levels of genes involved in the Wnt signaling pathway in CIRP silenced A549 cells. Red and blue indicate higher and lower gene expression, respectively, and the magnitude of expression differences is displayed by the color intensity. (C) qRT-PCR analysis the of mRNA levels of Wnt/β-catenin signaling pathway related genes was performed in CIRP-silenced A549 cells. Data were normalized to the levels in NS control A549 cells, and are shown as the means ± SD from three independent experiments. (D) Protein levels of the indicated genes in CIRP silenced A549 and H460 cells were determined by immunoblotting. (E) Protein levels of the indicated genes in CIRP-overexpressing H1299 and H1650 cells were determined by immunoblotting. (F) Protein levels of the indicated genes in CIRP-overexpressing and control A549 cells cotransfected with control or CTNNB1 siRNA for 48 h. (G) Protein levels of the indicated genes in CIRP-overexpressing and control A549 cells treated with IC50 of Triptonide for 48 h