Fig. 4

SLC2A1-AS1 depletion suppresses cell migration and invasion as well as glycolysis in ESCC cells. A. SLC2A1-AS1 downregulation suppresses cell migration and invasion in ESCC cells. B and C. Quantification of migrated cell numbers (B) and invasive cell numbers (C) in a variety of ESCC cells. D. SLC2A1-AS1 depletion suppressed EMT progression in ESCC cells. E. Quantification of E-cadherin, N-cadherin and Vimentin protein relative levels following treatment with NC or SLC2A1-AS1 siRNA. F. SLC2A1-AS1 knockdown suppresses Glut1 protein expression in a panel of ESCC cells. G. Quantification of Glut1 protein relative level following treatment with NC or SLC2A1-AS1 siRNA. H and I. SLC2A1-AS1 downregulation suppressed glucose consumption (H) and lactate production (I) in EC9706, TE1 and KYSE180 cells. J. GEO dataset GSE111011 assay for the expressions of glycolysis-related genes in 7 cases of ESCC samples and paired normal samples. K. SLC2A1-AS1 downregulation suppresses the expressions of glycolysis-related proteins, such as HK2, PFKM, PKM and LDHA, in ESCC cells. L. Quantification of relative expressions of HK2, PFKM, PKM and LDHA after transfection with NC or SLC2A1-AS1 siRNA in EC9706, TE1 and KYSE180 cells. Compared with NC group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance