Fig. 4
From: Chemoresistance is mediated by ovarian cancer leader cells in vitro
Ovarian cancer Leader cells represent a population of cells resistant to chemotherapy induced apoptosis. A LC-T2A-GFP cells were treated with the IC50 dose of cisplatin and sorted by flow cytometry. Cells were gated based on positive and negative controls where cisplatin treatment increased the percentage of the GFP+ LCs significantly. LC and FC percentages are expressed as the percentage of live cells. B LC-T2A-GFP cells were seeded as a monolayer on glass coverslips and treated with IC50 concentrations of cisplatin for 48 h. Cells were fixed and stained with the apoptosis maker, Cleaved Caspase-3 and imaged for GFP, Cleaved Caspase-3 and DAPI using the Cytation™ 3 Multimode Imager. C Data was analysed using the Gen5 software (V3.04, Biotek) to calculate the total cell counts, percentage of LCs (GFP+), apoptotic cells (cleaved caspase-3+) and double positive cell numbers. Statistical analysis was performed using a paired t-test; n = 4 focal areas per well. Scale bar at 200 μm