Fig. 2

Direct co-culture of chemotherapy-exposed MSC and breast cancer cells. (A) Flow cytometry analysis of gap junctions showed higher percentage of double-positive MDA and JIMT cells (red) in co-culture with DOX-/PAC-MSC compared to unexposed MSC. (B) Cytokine secretion analysis in tumor cell-MSC co-culture. (C) The effect of chemotherapy-exposed MSC on tumor cells proliferation analyzed by IncuCyte ZOOM™ kinetic imaging system. For statistical evaluation, the one-way ANOVA with Turkey´s or the Dunnett T3 multiple comparison test was used, where the cytokine production in unexposed MSC or tumor cells mixed with unexposed MSC was set as a control. * p < 0.05, ** p < 0.01, *** p < 0.001